Many popular museums, such as the San Diego Museum of Art, the San Diego Natural History Museum, the San Diego Museum of Man, and the Museum of Photographic Arts are located in Balboa Park. The Museum of Contemporary Art San Diego (MCASD) is located in an ocean front building in La Jolla and has a branch located at the Santa Fe Depot downtown. The Columbia district downtown is home to historic ship exhibits belonging to the San Diego Maritime Museum, headlined by the Star of India, as well as the unrelated San Diego Aircraft Carrier Museum featuring the USS Midway aircraft carrier.

San Diego has a growing art scene. "Kettner Nights" at the Art and Design District in Little Italy has art and design exhibitions throughout many retail design stores and galleries on selected Friday nights. "Ray at Night" at North Park host a variety of small scale art galleries on the second Saturday evening of each month. La Jolla and nearby Solana Beach also have a variety of art galleries.
The San Diego Symphony at Symphony Towers performs on a regular basis and is directed by Jahja Ling. The San Diego Opera at Civic Center Plaza, directed by Ian Campbell, was ranked by Opera America as one of the top 10 opera companies in the United States. Old Globe Theatre at Balboa Park produces about 15 plays and musicals annually. The La Jolla Playhouse at UCSD is directed by Christopher Ashley. Both the Old Globe Theatre and the La Jolla Playhouse have produced the world premieres of plays and musicals that have gone on to win Tony Awards or nominations on Broadway. The Joan B. Kroc Theatre at Kroc Center's Performing Arts Center is a 600-seat state-of-the-art theatre that hosts music, dance and theatre performances. The San Diego Repertory Theatre at the Lyceum Theatres in Horton Plaza produces a variety of plays and musicals. Other professional theatrical production companies include the Lyric Opera San Diego and the Starlight Musical Theatre.
Hundreds of movies and a dozen TV shows have been filmed in San Diego, a tradition going back as far as 1898.